Description
Description: This assay employs a two-site sandwich ELISA to quantitate APOA1 in samples. An antibody specific for APOA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Apolipoprotein A-I the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters.
ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.ApoA-1 Milano is a naturally occurring mutant of ApoA-I, found in a family descended from a single couple of the 18th century